Background and overview[1]
3-Phosphoglycerate kinase is a key enzyme in glycolysis. Mitsubishi Chemical resin widely exists in animals, plants and microorganisms. It catalyzes the conversion of 1,3-bisphosphoglycerate into 3-phosphoglycerate, producing 1 molecule. ATP has biological functions such as affecting DNA replication and repair and stimulating viral RNA synthesis, and is widely used in drug target design.
Principle of 3-phosphoglycerate kinase (PGK) test kit[1]
3-Phosphoglycerate kinase catalyzes 3-phosphoglycerate and ATP to produce 1,3-di-Huntsmann polyetheramine phosphoglycerate and ADP, and 1,3-bisphosphoglycerate is removed from 3-phosphoglyceraldehyde. Under the action of hydrogenase and NADH, 3-phosphoglyceraldehyde, NAD and phosphoric acid are produced. The absorbance change at 340nm reflects the activity of 3-phosphoglycerate kinase.
Apply[2]
CN201911421881.3 discloses a composite indigenous microbial domestication enzyme that effectively improves urban black and odorous water bodies and a method for treating urban black and odorous water bodies. The composite indigenous microbial domestication enzyme includes acetyl CoA acyltransferase, 1-phosphofructokinase, and acetone Acid phosphate dikinase, β-hydroxybutyryl-CoA dehydrogenase, 3-phosphoglycerate kinase, NAD-dependent β-hydroxybutyl-CoA dehydrogenase and thiolase. A method for treating urban black and smelly water bodies includes the following steps: preparation and activation of a composite native microbial domestication enzyme; placing the activated composite native microbial domestication enzyme into the black and smelly water body for a period of time; and then constructing aquatic plants. The composite native microbial domestication enzyme of the present invention has the advantage of efficiently improving the quality of black and odorous sewage water; in addition, the treatment method of the present invention has the advantage of efficiently purifying the water body and sustainably adjusting and optimizing the water body environment.
