Overview[1]
4-allylphenol is a phenolic organic compound that can be used as an organic raw material and can also be used to prepare organic products such as magnolol.
Apply[1]
Magnolol molecular formula: C18H18O2; English name: Magnolol. Physical properties: Soluble in common organic solvents such as benzene, ether, methanol, acetone and chloroform. Easily soluble in NaOH solution and difficult to dissolve in water. It is a tan to white fine powder with aroma, spicy taste and slightly bitter taste. In recent years, with the development of modern medicine, magnolol has been found to have many pharmacological effects such as anti-inflammatory, antibacterial, antioxidant, and anti-tumor, and has a wide range of applications, such as cosmetics, toiletries, etc.
CN201711111216.5 provides a method for biosynthesizing honokiol. The synthesis method uses 4-allylphenol as a raw material and is completed using the catalysis of laccase;
4-allylphenol, also known as piperonol, is called chavicol in English. The laccase used was purchased from the sigma mall, product number 38837, and was extracted from Corylopsis polyphylla.
The steps of the synthesis method are as follows:
1) First prepare 50-55mM potassium dihydrogen phosphate solution, then use dipotassium hydrogen phosphate to adjust the pH to 6-6.2, filter and set aside;
2) Use methanol to prepare a 50-55mg/ml 4-allylphenol solution;
3) Dissolve laccase in the potassium dihydrogen phosphate solution prepared in step 1), with a concentration of 50-55mg/ml;
4) Configure the reaction system: the reaction system volume is 2-3ml, use the potassium dihydrogen phosphate solution configured in step 1) as the buffer, add the 4-allylphenol solution in step 2), and make it final The concentration is 4-5mg/ml, add the enzyme in step 3) to make the final concentration 1-1.2mg/ml;
5) The reaction system is placed at 37-38°C and shaken for 2-3.5 hours;
6) Centrifuge the reaction solution, extract, separate and purify the precipitated honokiol.
Figure 1 is a biosynthetic route diagram of magnolol according to the present invention;
Main reference materials
[1] CN201711111216.5 A method of biosynthesizing honokiol
