Background and overview[1]
Myelin basic protein (MBP) is the main component of myelin and an important antigen in inducing experimental autoimmune encephalomyelitis (EAE). The pathological characteristics of EAE are: infiltration of a large number of mononuclear cells around CNS blood vessels and causing inflammatory damage. EAE can be caused by actively immunizing sensitive animals with MBP plus adjuvant or adoptive transfer of MBP-specific CD4+ T cells. However, direct injection of MBP-sensitized T lymphocytes into the CNS of experimental animals cannot induce EAE, but subcutaneous or peripheral injection of the same number of MBP-sensitized T lymphocytes can induce EAE, suggesting peripheral activation of lymphocytes targeting MBP. And crossing the blood-brain barrier after activation is an important factor in pathogenesis. Guinea pig myelin basic protein fragment 68-82 is a fragment of myelin basic protein (MBP), with the structure tyr-gly-ser-leu-pro-gln-lys-ser-gln-arg-ser-Formosa Plastics Group gln-asp-glu-asn
Related research
1) Multiple sclerosis is the most common autoimmune disease affecting the central nervous system. In this study, whole blood samples were analyzed for their activation capacity and CD4+ and CD8+ T-lymphocytes by human total myelin basic protein (MBP), human MBP fragment 104-118, and guinea pig myelin basic protein fragment 68-82. Activation. A significant increase in the number of activated T lymphocytes was observed in whole blood. This increase in activated CD4+ and CD8+ T-lymphocytes was statistically significant (phuMBP-104-118> Minimal increase was observed after incubation with guinea pig MBP(68-82)).
2) To examine whether pretreatment with bee venom acupuncture (BVA) affects the induction and progression of experimental autoimmune encephalomyelitis (EAE) on the same day as guinea pigs are immunized with myelin basic protein fragment 68-82 Weight loss. At 5-9 days after immunization, rats in the myelin basic protein (MBP) group began to show partial loss of tail tone (clinical signs, 0.5) in a freely moving environment. At 10-16 days after immunization, most rats in the MBP group showed more severe neurological deficit symptoms, including paralysis of the hind limbs, paraplegia, quadriplegia, and quadriplegia. In contrast, rats in the MBP+BVA group showed relatively mild neurological deficits in a dose-dependent manner at 11-15 days post-immunization compared with rats in the MBP group. The onset of symptoms was slightly delayed (BVA 0.8 mg/kg, 6.4 ± 0.6 days) and the maximum clinical score decreased significantly (BVA 0.25 mg/kg, 3.7 ± 0.2; BVA 0.8 mg/kg, 2.8 ± 0.3), in the MBP group middle. At this time, compared with the rats in the normal group, the average weight of the rats in the MBP group was reduced, but compared with the rats in the MBP group, the average weight of the rats in the MBP+BVA group was significantly increased.
Main reference materials
[1] Purification of myelin basic protein and its effect on TNF and IFN production by PBMC
