Background and overview[1][2]
D-glucose-6-phosphate disodium is an organic substance, which is mainly used in biochemical research to determine the substrate of glucose-6-phosphate dehydrogenase. If D-glucose-6-phosphate disodium is inhaled, move the patient to fresh air; if skin contact occurs, take off contaminated clothing, rinse the skin thoroughly with soap and water, and seek medical attention if you feel unwell; if the eye contact If exposed to sunlight, separate eyelids, rinse with running water or saline, and seek medical attention immediately; if ingested, rinse mouth immediately, do not induce vomiting, and seek medical attention immediately. Advice to protect rescuers is as follows: Move the patient to a safe place, consult a doctor, and if conditions permit, please show this chemical safety data sheet to the doctor who comes to the scene. If there is a small leak, collect the leaked liquid in a sealable container as much as possible, absorb it with sand, activated carbon or other inert materials, and transfer it to a safe place. Do not flush it into the sewer; if there is a large leak, build a dike or dig a pit. Contain, seal the drainage pipe, cover it with foam to inhibit evaporation, use an explosion-proof pump to transfer it to a tanker or a special collector, and recycle or transport it to a waste treatment site for disposal.
Structure
Apply[1-2]
D-glucose-6-phosphate disodium is mainly used in biochemical research.
Measurement of substrates for glucose-6-phosphate dehydrogenase. Examples of its application are as follows:
1. Used for the identification of metabolites of epiberberine in vitro in rat liver microsomes. Epiberberine is an important isoquinoline alkaloid in Coptis chinensis. Studies have shown that epiberberine has strong antioxidant activity, can inhibit aldose reductase, lower blood sugar, and combat potential complications of diabetes, and its effect is stronger than berberine. D-glucose-6-phosphate disodium and glucose-6-phosphate dehydrogenase used in the detection process were purchased from Beijing Bayerdi Biotechnology Co., Ltd. (packaged by Sigma Company). D-glucose-6-phosphate disodium is mainly used for body temperature incubation of liver microsomes of probe drugs during the detection process: preheat each probe drug with RLM in a water bath at 37 ℃ Incubate for 5 minutes, then add NADPH generating system (final concentration contains 1 mmol/L NADPNa2, 20 mmol/LD-glucose-6-phosphate disodium (G-6-P), 2 U/L G-6-PDH, 20 mmol/L MgCl2), dilute to volume with 0.15 mol/L KCl buffered gas-phase silica solution (pH 7.4) so that the final volume of the entire incubation system is 0.4 mL (containing metoprolol 20 μmol/L, dapsone 25 μmol/L, phenacetin 25 μmol/L, chlorzoxazone 25 μmol/L, tolbutamide 25 μmol/L) and 1 g/L protein in 37 ℃ water bath Shake evenly for 40 minutes. Take out the incubated sample in vitro and put it into an ice bath. Add ice-cold methanol according to 1∶3 to terminate the reaction. At the same time, add 20 μL of internal standard thixotropic agent 0.06 g/L Schisandrin Methyl and shake for 30 s, centrifuge at 10,000 × g for 20 min at 4 ℃. Aspirate the supernatant and blow dry with nitrogen, reconstitute the volume with methanol (μL), 4 ℃, 10,000 × g, centrifuge for 20 minutes, inject 20 μL of the supernatant, and perform HPLC analysis. Each sample is analyzed. 3 repeats.
2. Used to determine the inhibitory effect of evodiacarpine on the liver metabolism of five Coptis alkaloids in vitro in rats.
Coptis chinensis and Evodia rutaecarpa have been used together for a long time. The two medicines, one cold and the other hot, complement each other in opposite directions. The main components of coptis are isoquinoline alkaloids, including coptisine (COP), epiberberine (EPI), berberine (BER), palmatine (PAL), jatrorrhizine (JAT), etc. Alkali (RUT) is the main component of Evodia Fructus. Research in recent years has found that there is an obvious pharmacokinetic interaction between the components of Coptidis Coptidis and Evodia Fructus. Coptidis alkaloids are mainly metabolized in the liver. RUT in the components of Evodia can selectively inhibit the activity of rat and human liver microsomal enzymes. The components of Evodia have an inhibitory effect on the liver metabolism of rats with BER. Since different coptis alkaloids have obvious intestinal absorption selectivity, it is speculated that Evodia Fructus should have an inhibitory effect on the hepatic metabolism of each coptis alkaloid and be selective. An experiment used a rat in vitro liver microsome body temperature incubation metabolism model to determine the effect of RUT on five alkaloid components (COP, EPI, BER, PAL, JAT) in Coptis chinensis by obtaining the half inhibitory concentration (IC50) and inhibition constant (Ki) values. Whether it has in vitro metabolic inhibitory effect and its intensity, compare the effect of RUT on five kinds of organisms.Differences in alkali inhibitory effects. D-glucose-6-disodium phosphate (G-6-P) is mainly used for the preparation of NADPH-generating system during the detection process: accurately weigh appropriate amounts of NADPNa2, G-6-P, G-6-PDH, and MgCl2, and add water After quantitative dissolution, a NADPH-generating system containing 2 mmol/L NADPNa2, 20 mmol/L G-6-P, 4 U/L G-6-PDH, and 40 mmol/L MgCl2 was obtained.
Main reference materials
[1] Yang Xiaoyan, Ye Jing, Sun Guixia, et al. Identification of metabolites of epiberberine in vitro in rat liver microsomes and its inhibitory effect on CYP2D6 enzyme activity [J]. Chinese Journal of Traditional Chinese Medicine, 2014, 39(19 ): 3855-3859.
[2] Xue Baojuan, Li Zhihui, Zhang Yujie, et al. Inhibitory effect of evodiacarpine on the in vitro liver metabolism of five Coptis alkaloids in rats [D]. , 2014.
