Background[1][2]
Heat shock protein (HSP) is a type of protein produced under the induction of various stress factors, especially high-temperature environmental factors. HSPs-peptide complexes are expected to be specific by activating multiple CTL clones in the body. Kill all tumor cells in the same tumor, break immune tolerance, eliminate residual tumor tissue, and overcome the difficulties such as antigen variability and cell heterogeneity that are currently difficult to solve in tumors. According to their different molecular weights, they can be divided into HSP110, HSP90, HSP70, HSP60 and small molecule heat shock protein subfamilies.
CDC37, as an important auxiliary molecular chaperone of heat shock protein 90 (HSP90), can specifically bind to protein kinases and prevent misfolding and degradation of newly synthesized protein kinases. Studying fumed silica found that the expression level of CDC37 in actively proliferating tissues was significantly higher than that in normal tissues, and overexpression of CDC37 can promote the proliferation of normal prostate epithelial cells.
The expression of CDC37 in liver cancer tissue is significantly higher than that in peritumoral tissue and normal liver tissue. Silencing CDC37 gene expression in liver cancer cell lines will significantly inhibit the formation and growth of tumor models in nude mice. During the formation of liver cancer, The high expression of CDC37 can weaken the inhibition of cell growth by P16, suggesting that the upregulation of CDC37 expression plays an important role in the occurrence of HCC. Monoclonal antibody technology originated from the in vitro mouse hybridoma technology created by Kohler et al.
After years of research, mouse monoclonal antibodies have developed from simple biological laboratory research and clinical diagnosis to therapeutic antibody drugs. However, the main drawback of mouse monoclonal antibodies is that the mouse immune system cannot recognize certain immunogens, especially murine-derived immunogens, and the affinity of mouse monoclonal antibodies is not as high as that of rabbit-derived antibodies. However, due to the lack of rabbit-derived plasmacytoma and the technical difficulties in transfecting rabbit B cells with viruses in vitro, it was not until 1995 that a rabbit plasmacytoma cell line was successfully obtained from c-myc/v-abl transgenic rabbits, and the Stable rabbit-rabbit hybridomas led to a breakthrough in rabbit monoclonal antibody technology. In recent years, rabbit monoclonal antibody technology has been improving day by day, and there are currently thousands of commercialized rabbit monoclonal antibodies.
Phosphorylated CDC37 (Ser13) rabbit monoclonal antibody is stored at -20℃, and Western primary antibody diluent is stored at -20℃ or 4℃. It is valid for one year. It is recommended to store the Western primary antibody dilution at 4°C. If not used for a long time, storage at -20°C may be considered. However, freezing and thawing may cause slight turbidity and a small amount of insoluble matter.
If Phospho-CDC37 (Ser13) Rabbit Monoclonal Antibody is used for Western blot (WB, 1:1000 – 1:10000), immunofluorescence (IF), immunocytochemistry (ICC, 1:10 – 1:100) , Flo (1:10 – 1:100), IHC-P (1:250 – 1:500), IP (1:10 – 1:100), IHC water-based amino resin-Paraffin (1:250 – 1:500 ), Flow Cytome try (1:10-1:100) and other experiments, please pay attention to recycling the used diluted antibodies. Recycled phospho-CDC37 (Ser13) Rabbit mAb can usually be reused at least 5-10 times.
Please store diluted phospho-CDC37 (Ser13) rabbit monoclonal antibody at 4°C, including diluted antibodies that have been used. Antibodies that are reused after recovery can be used in the same way as freshly diluted antibodies. If you find that the antibody is slightly turbid during repeated use, you can centrifuge it at 10,000g for 1-3 minutes and take the supernatant for subsequent testing. If the recovered antibody shows obvious floc or mold growth, you can consider discarding the antibody.
Main reference materials
[1] Li Tingting, Cui Huifei. Development and application prospects of rabbit monoclonal antibodies [J]. Food and Drugs, 2012, 14(9): 373-376.
[2] HBV replication and different gene expression do not affect CDC37 levels
