Background and overview[1]
Tributyl ammonium pyrophosphate is an organic intermediate, which can be obtained from the reaction of tetrasodium pyrophosphate and n-tributylamine. There are reports in the literature that it can be used to prepare a base-modified nucleotide.
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Preparation[1]
Tetrasodium pyrophosphate (26.6g, 0.1mol) was dissolved in deionized water (500mL). The aqueous solution was passed through a strong ion exchange column (pk216 proton type). The fractions containing pyrophosphate were combined and used with n-tributylamine. (37.1g, 0.2mol) to form a salt, and the above eluent was concentrated into oil using a rotary evaporator. The oily substance was treated with water through 1,4-dioxane (100 mL), and the watering was repeated three times to obtain the oily substance tributyl ammonium pyrophosphate (quantitative).
Apply[2]
CN201310533070.9 discloses a method for synthesizing base-modified nucleotides and its use; the method includes using various modified nucleosides under the action of a phosphorylation reagent to selectively generate 5′-nucleosides Triphosphoric acid; these phosphorylation reagents include cyclic phosphorylation reagents generated in situ during the reaction and the reaction products of the synergistic action of phosphorus oxychloride and tributyl ammonium pyrophosphate. The base-modified nucleotide synthesized by the present invention is widely used in biological fields such as DNA sequencing, labeling, and extension. Currently, its sales price is very high, the synthesis method is complex, the reaction selectivity is poor, the side reactions are many reverse osmosis bactericides, and it is difficult to separate. Purification; and the synthesis method provided by the present invention has good selectivity, few side reactions, is easy to separate and purify, and the required experimental conditions are easy to obtain. The synthesis process is a conventional chemical reaction and can be used for large-scale promotion and use.
