Background[1-3]
Choline phosphatase 2 antibody is a polyclonal antibody that can specifically bind to choline phosphatase 2 protein. It is mainly used for in vitro immunological detection of choline phosphatase 2.
Cholinesterase is a type of glycoprotein that exists in the body in the form of multiple isoenzymes. Generally it can be divided into true cholinesterase and pseudocholinesterase. True cholinesterase, also known as acetylcholinesterase, mainly exists in the synaptic cleft of cholinergic nerve terminals, especially in the folds of the postsynaptic membrane of motor nerve end plates; it also exists in cholinergic Within neurons and in red blood cells. This enzyme has the strongest effect on physiological concentrations of ACh and has high specificity. One enzyme molecule can hydrolyze 3×10 molecules of ACh, and is often referred to as cholinesterase. Pseudocholinesterase is widely present in glial cells, plasma, liver, kidney, and intestine. Less specific for ACh, pseudocholinesterase hydrolyzes other choline esters such as succinylcholine.
Choline phosphatase 2 immunohistochemistry
The active center on the surface of the cholinesterase protein molecule has two sites that can bind acetylcholine, namely the negatively charged anion site and the ester hydrolysis site. The esterification site contains an acidic site composed of the hydroxyl group of serine and a basic site composed of the imidazole ring of histidine. The two are combined through hydrogen bonds, which enhances the nucleophilic activity of the hydroxyl group of serine, making it easy to combine with acetylcholine. combine.
The process of cholinesterase hydrolyzing acetylcholine can be divided into three steps: ① The positively charged quaternary ammonium cation head in the acetylcholine molecular structure combines with the anionic site of cholinesterase through electrostatic attraction; at the same time, the acetylcholine molecule The carbonyl carbon is covalently bonded to the hydroxyl group of serine at the esterolysis site of cholinesterase to form a complex of acetylcholine and cholinesterase. ②The acetylcholine and cholinesterase complex is cleaved into choline and acetylcholinesterase. ③Acetylcholinesterase rapidly hydrolyzes, separates acetic acid, and restores enzyme activity.
Apply[4][5]
Study on the biological activity and mechanism of action of mugwort essential oil on diamondback moth
To study the biological activity of Artemisia lavandulaefolia DC. essential oil on the diamondback moth [Plutella xylostella (L.)], and the effect of Artemisia lavandulaefolia essential oil on the activity of several enzymes in the diamondback moth larvae.
The results are as follows: 1. Wild mugwort essential oil has a good contact killing effect on the third instar larvae of diamondback moth. And within the same period of time, as the dose increases, the effect becomes more obvious.
2. Mugwort essential oil has a certain stomach poisoning effect on the third instar larvae of diamondback moth. Under the same concentration treatment, the stomach poisoning effect of mugwort essential oil on diamondback moth larvae increased rapidly over time, especially after 24 hours, the death rate of test insects increased rapidly.
3. Mugwort essential oil has certain fumigation activity against diamondback moth larvae. The corrected mortality rate for fumigation activity of diamondback moth larvae increased with increasing concentration during the same period of time. Treated at the same concentration, the corrected mortality rate for fumigation activity of diamondback moth larvae gradually increased with time.
4. Mugwort essential oil has a very obvious fumigation effect on diamondback moth adults. After 1 hour of treatment, the corrected mortality rate of the 0.4 μL/L treatment group was 86.67%. After 4 hours of treatment, the corrected mortality rate of the 0.4 μL/L treatment group reached 100%.
5. The repellent rate of mugwort essential oil on diamondback moth adults increases with the increase in dose. The repellent rate of diamondback moth treated with various doses of mugwort essential oil reached the highest level V.
6. Mugwort essential oil has an inhibitory effect on acetylcholinesterase activity, acid phosphatase activity, alkaline phosphatase activity and carboxylesterase activity in diamondback moth larvae. Mugwort essential oil first activates and then inhibits glutathione-S-transferase activity and peroxidase activity in diamondback moth.
