Background[1-3]
Creatine phosphokinase 2 antibody is a type of polyclonal antibody that can specifically bind to creatine phosphokinase 2. It is mainly used in immunological experiments related to the in vitro detection of creatine phosphokinase 2.
Creatine phosphokinase (CK) is an enzyme widely present in muscle tissues such as skeletal muscle and cardiac muscle. It can catalyze the following reversible reaction: creatine ≒ creatine phosphate. Through the catalysis of CK, creatine combines with ATP to decompose a phosphate group released to generate creatine phosphate; under the catalysis of CK, creatine phosphate is converted into creatine to decompose a high-energy phosphate bond and release a phosphate group to supply energy. , so creatine phosphate is a form of energy storage in the body, and CK is an enzyme that catalyzes this energy conversion. During myocardial infarction, CK can be significantly increased, but the factors that can cause CK to increase are not just myocardial infarction. Progressive muscular dystrophy, polymyositis, muscle damage, muscular dystrophy, alcoholism, hypothyroidism, pulmonary infarction, cerebrovascular disease, organophosphorus pesticide poisoning and cardiac surgery can all cause elevated CK.
Creatine phosphokinase 2 antibody
CKMT2 (creatine kinase, mitochondrial 2 (sarcomere)) is a creatine kinase isoenzyme that (reversibly) catalyzes the production of ATP from ADP by converting creatine phosphate to creatine. Creatine phosphate serves as an energy store for rapid ATP production. There are two isoenzymes of mitochondrial creatine kinase: creatine phosphate mitochondrial creatine kinase (CKMT2) and ubiquitous creatine kinase, which are encoded by separate genes.
Mitochondrial creatine kinase exists in two different oligomeric forms: dimers and octamers, while there is only a dimeric cytosolic creatine kinase isoenzyme. Sarcomeric mitochondrial creatine kinase shares 80% homology with the coding exons of the ubiquitous mitochondrial creatine kinase. The CKMT2 gene contains sequences homologous to several motifs shared by some nuclear genes encoding mitochondrial proteins and may therefore be necessary for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been discovered.
Apply[4][5]
Clinical study on the relationship between creatine phosphokinase and hypertension in youth
Clinical study to explore the relationship between serum creatine phosphokinase levels and essential hypertension in young people.
Methods: 120 young patients with essential hypertension were collected as the hypertension research group (77 males, 43 females, average age (34.50±5.89 years)), and 60 healthy people who had physical examination were selected as the control group (males) 35 cases, 25 women, average age (35.50±4.89 years)).
According to the World Health Organization (WHO) hypertension classification standards, it is divided into: level 1 group (n=20), level 2 group (n=42), and level 3 group (n=58); according to the WHO hypertension risk classification The stratum standards are divided into: low-risk group (n=15), intermediate-risk group (n=25), high-risk group (n=32), and very high-risk group (n=48). All selected cases had blood drawn to test serum CK, blood lipids, blood sugar, liver and kidney function and other biochemical indicators.
Compare the serum CK levels between the hypertension group and the control group, as well as the differences in serum CK levels in the hypertension group in different grades and risk stratifications; at the same time, perform correlation analysis on serum CK and various cardiovascular disease risk factors.
Results 1. There was no statistically significant difference in age, gender, TC, TG, LDH-C, HDL-C, GLU, and CRE between the two groups of research subjects (P>0.05); high The serum CK, UA, SBP, DBP, and BMI in the blood pressure group were higher than those in the control group, and the difference was statistically significant (P<0.05).
2. Comparison of serum CK levels in different grades of hypertension: Comparing the general information of the three groups with different grades of hypertension, the difference is not statistically significant (P>0.05); comparing the three groups in SBP and DBP, the difference is statistically significant ( P<0.05). The serum CK in the grade 2 group (145.19±57.44U/L) was higher than that in the grade 1 group (112.00±52.45U/L), and the difference was statistically significant (P<0.05). It was higher than the serum CK in the grade 3 group (231.07±67.12 U/L) was low, and the difference was statistically significant (P<0.05).
3. Comparison of serum CK levels in different risk stratifications of hypertension: Comparison of general information and antioxidants among the four groups of hypertension in different risk stratifications, the difference was not statistically significant (P>0.05); the four groups in terms of SBP and DBP , the difference in solvents is statistically significant (P<0.05). Serum CK in the intermediate-risk group (141.52±53.90U/L) was significantly higher than that in the lower-risk group (100.53±53.38U/L), and the difference was statistically significant (P<0.05), while in the higher-risk group (181.56±84.71U/L) L) was significantly reduced, and the difference was statistically significant (P<0.05). Compared with the high-risk group, the very high-risk group (226.75±60.18U/L) had a significantly higher serum CK, and the difference was statistically significant (P<0.05).
