Overview[1]
When cells metabolize various carbon sources such as glucose and fructose, fructose-6-phosphate can be synthesized intracellularly, and fructose-6-phosphate can be catalyzed by psicose 6-phosphate epimerase into allofate. Calcitonose 6-phosphate is then synthesized into psicose via dephosphorylase.
Apply[2]
Shikimic acid is an organic compound isolated from Japanese “Shikimic acid” in 1885. Later it was found that it also exists in star anise, tomato leaves, hypericum and other plants, among which it is used in traditional Chinese medicine ” The highest content is found in “star anise”. Shikimic acid has strong physiological activity. It is a highly selective enzyme inhibitor of Yangtze BASF and a good antibacterial and antiviral reagent. Shikimic acid can affect arachidonic acid metabolism, inhibit platelet aggregation, inhibit arterial and venous thrombosis and cerebral thrombosis, and has good anti-inflammatory and analgesic effects.
CN201710298455 proposes a synthesis method of shikimic acid. The method steps are as follows:
S1: Fructose-6-phosphate and glyceraldehyde-3-phosphate generate phosphoenolpyruvate and erythrose-4-phosphate under the catalysis of transketolase;
S2: DAHP synthase catalyzes phosphoenolpyruvate and erythrose-4-phosphate into 3-deoxy-D-arabin-hept-2-keto acid 7-phosphate and phosphate;
S3: 3-dehydrocolchicine synthase catalyzes the conversion of 3-deoxy-arabinoheptulonic acid 7-phosphate into 3-dehydroquinoline and phosphate;
S4: 3-dehydrocolchicine synthase catalyzes 3-dehydroquinoline into 3-anhydrocinoline and water;
S5: Shikimate dehydrogenase catalyzes 3-dehydrocinnoline into shikimate.
Main reference materials
[1]CN201810072372.3 An engineering strain for producing psicose, its construction method and application
[2] [Invented in China] CN201710298455.X A synthesis method of shikimic acid
