Background and overview[1]
L-Ascorbic acid-2-phosphate trisodium salt in Chinese is also known as vitamin C phosphate sodium, SAP, L-ascorbic acid-2-phosphate sodium salt. L-Ascorbic acid-2-phosphate trisodium salt can be used as a pharmaceutical and chemical synthesis intermediate. If L-ascorbic acid-2-trisodium phosphate is inhaled, move the patient to fresh air; if there is skin contact, take off contaminated clothing, rinse the skin thoroughly with soap and water, and seek medical attention if you feel unwell; if In case of eye contact, separate eyelids, rinse with running water or saline, and seek medical attention immediately; if ingested, rinse mouth immediately, do not induce vomiting, and seek medical attention immediately.
Apply[1-2]
L-Ascorbic acid-2-phosphate trisodium salt can be used as a catalytic substrate for alkaline phosphatase. Alkaline phosphatase (ALP) is an important biomarker, and its abnormal expression is associated with various diseases such as breast cancer, prostate cancer, bone disease, diabetes, and liver dysfunction. Quantitative detection of ALP in serum can assist in the diagnosis of related diseases. Spectral analysis method has been widely used in ALP detection due to its advantages of simplicity and fast response. However, conventional spectroscopic methods based on a single material and with a single colorimetric or fluorescence output signal have poor anti-interference capabilities and low accuracy, making it difficult to detect targets in complex samples. With the advancement of nanotechnology, a variety of ALP colorimetric sensing methods based on nanogold and nanosilver have been developed, but the sensitivity of such methods needs to be improved; weak shell changes of core-shell structured nanoparticles can cause significant The emergence of spectral and color changes provides the possibility to further improve the sensitivity of colorimetric detection of carbon black. Some studies have provided a dual-channel detection method for alkaline phosphatase activity. The principle is that alkaline phosphatase catalyzes the hydrolysis of its substrate L-ascorbic acid-2-trisodium phosphate (AAP) to generate reducing ascorbic acid, and ascorbic acid is reduced Tollen’s reagent generates silver elements and deposits them on the surface of gold nanoparticles to form Au@AgNPs, causing changes in the color and absorption spectrum of the solution; in addition, the generated Au@AgNPs can effectively quench the fluorescence of graphene quantum dots (GQDs), causing the solution to Changes in fluorescence intensity. Thus, colorimetric and fluorescent dual-channel measurement of alkaline phosphatase activity can be achieved through simultaneous changes in solution color, absorbance, and fluorescence intensity.
L-Ascorbic acid-2-phosphate trisodium salt can also be used in the preparation of a photoelectrochemical parathion sensor. It belongs to the field of new nano functional materials and biosensor technology. First, a new two-dimensional nanophotosensitive material Mn-MoO3/TiO2@g-C3N4 was prepared. The good biocompatibility and large specific surface area of the solid saturated polyester resin of this material were used to load parathion antibodies, and then Alkaline phosphatase is fixed by cross-linking with glutaraldehyde. During detection, alkaline phosphatase can catalyze L-ascorbic acid-2-trisodium phosphate AAP to produce L-ascorbic acid AA in situ, and then provide photoelectric detection. Provide an electron donor, and then use the impact of the specific quantitative combination of the antibody and the antigen on the electron transmission ability to reduce the photocurrent intensity accordingly. Finally, a detection pair with low cost, high sensitivity, good specificity, rapid detection, and simple preparation is produced. Label-free photoelectrochemical biosensor for phosphorus.
Main reference materials
[1] CN201811229090.6 Dual-channel detection method of alkaline phosphatase activity
[2] CN201610102027.0 Preparation method and application of photoelectrochemical parathion sensor based on two-dimensional nanophotosensitive materials
