Background and overview[1][2]
Beta-glycerophosphate sodium pentahydrate is also known as beta-glycerophosphate pentahydrate and beta-GP in Chinese. Beta-glycerophosphate sodium pentahydrate can be used as a substrate for calf myo-inositol-1-phosphatase in biochemical studies and for the determination of phosphatase in blood.
Structure
Apply[1-2]
Beta-glycerophosphate sodium pentahydrate can be used as a substrate for biochemical research, calf myo-inositol-1-phosphatase, and determination of phosphatase in blood. Examples of its application are as follows:
1. Used to prepare capsule-shaped chitosan gel beads that can contain small molecule dyes.
The sugar-sodium glycerophosphate thermosensitive gel system was first seen in 2000. Once it was proposed, it attracted great attention. First of all, the system uses the natural polymer material chitosan, and its biocompatibility and biodegradability have gradually improved. Researcher Approval.
In addition, simple chitosan only remains in solution in an acidic environment. When the pH value rises to 6.9-7.1, the chitosan solution begins to precipitate, but with the participation of weak base sodium glycerophosphate, the system It can maintain a stable solution state under neutral conditions, which provides convenient conditions for loading protein drugs and even living cells into the system. Finally, the system has temperature-sensitive characteristics and is in a solution state below room temperature. When the temperature rises to body temperature, it solidifies to form a gel, thereby releasing the effect of the loaded drug.
However, if the chitosan-sodium glycerophosphate thermosensitive gel system is used to directly load protein drugs or even living cells, there are still several problems: 1) The shape of the gel is uncertain. 2) Due to the unsealed nature of the gel, small molecules such as drug dyes and other small molecules contained in the gel may break away from the gel during the transfer process. 3) The mechanical strength of a single gel-encapsulated drug is very poor, and the encapsulated structure is easily damaged. In response to the above problems, some research has developed a capsule-shaped gel bead with closed properties and a certain shape.
The preparation method is as follows: dissolve chitosan in an acetic acid solution, and slowly add β-glycerophosphate sodium pentahydrate solution dropwise in an ice-water bath. And adjust the pH value of the mixed solution to neutral. The prepared chitosan/β-sodium glycerophosphate pentahydrate mixed solution (CS/β-GP solution) is dropped into the SDS receiving solution, and after maturation, chitosan droplets surrounding the CS/β-GP solution are formed. After heating in a water bath, gelation occurs inside due to hydrogen bonding, and chitosan gel beads are finally obtained. The prepared chitosan gel beads have excellent encapsulation properties for small molecule dyes with molecular weights between 300 and 800. The amount of dye leakage is small and the encapsulation stability is good. There is still no obvious leakage after 12 hours. The specific preparation process is as follows:
1) Preparation of chitosan/β-sodium glycerophosphate pentahydrate mixed solution: Dissolve 1.2g-1.6g β-sodium glycerophosphate pentahydrate in 2mL of water, and add 0.4g of medium viscosity chitosan Dissolve in 0.1 mol/L acetic acid solution; slowly add β-glycerophosphate sodium pentahydrate solution to the dissolved chitosan solution under an ice water bath to obtain chitosan/β-glycerophosphate sodium pentahydrate. Hydrate mixed solution, abbreviated as CS/β-GP mixed solution;
2) Adjustment of pH value of chitosan/β-glycerol sodium phosphate pentahydrate mixed solution and preparation of sodium lauryl sulfate solution: Weigh 25g of disodium hydrogen phosphate solid and dissolve it in 100ml of water, and dissolve until saturated Disodium hydrogen phosphate solution; add saturated disodium hydrogen phosphate solution dropwise to the CS/β-GP mixed solution to adjust the pH value to between 6.9-7.1; weigh 0.2g-0.8 sodium lauryl sulfate solid and dissolve in In 20mL of water, prepare a sodium dodecyl sulfate receiving solution with a mass fraction of 1%-4%, which is referred to as SDS receiving solution;
3) Preparation of chitosan gel beads: Use a pipette to drop the CS/β-GP solution with a pH value of 6.9-7.1 into the SDS receiving solution, and obtain chitosan microcapsules after maturation. Then wash it three times with distilled water, and then put it into a water bath for heating. The CS/β-GP solution inside the microcapsule transforms into a gel, thereby obtaining chitosan gel beads.
2. Used to prepare a new type of composite biomimetic hydrogel.
The material described is β-tricalcium phosphate/chitosan composite biomimetic hydrogel. First, dissolve chitosan with dilute acetic acid solution, dissolve sodium β-glycerophosphate (β-GP) in triple distilled water, and��The above two solutions were stored in a 4°C refrigerator overnight; add β-tricalcium phosphate to the chitosan solution under continuous stirring, bathe in ice, add β-GP solution, and add a small amount dropwise in a neutral pH environment. Glyoxal, place the mixed solution at 37°C overnight to form a hydrogel, and store the product in a 4°C refrigerator to obtain the β-tricalcium phosphate/chitosan composite biomimetic hydrogel material.
The materials provided overcome the application limitations of existing bone repair materials when rapid bone filling is required, the environment of the bone tissue repair site is complex, or minimally invasive treatment is required. The preparation method is simple, low-cost, and synthetic. It is safe and has important application value in the field of biomedical engineering.
Main reference materials
[1]CN20161117 Shanghai Chemical Industry Co., Ltd. 6504.4 A preparation method of chitosan gel beads
[2]CN201611204019.3 Preparation method of a new type of β-tricalcium phosphate/chitosan composite biomimetic hydrogel
